Programme
CRISPR 2021
June 1-10, 2021

 

 

              

 
Tuesday, June 1, 2021
Session 1
4.00 pm-8.00 pm (GMT+2)
Chair: Hynes Alexander, McMaster University, Canada
0
4.00 pm (GMT+2)

Opening remarks


1
4.15 pm (GMT+2)

Keynote: Spacer acquisition: scavenging for dead DNA to collect infection souvenirs

Luciano Marraffini
Rockefeller University, United States
2
5.00 pm (GMT+2)

A flexible seed in type III CRISPR-Cas allows for within-host spacer diversity

J. Steens*1, Y. Zhu1, D. Taylor2, J. Bravo2, S. Prinsen3, C. Schoen1, B. Keijser4, M. Ossendrijver4, M. Hofstra5, S. Brouns6, A. Shinkai7, J. Van Der Oost1, R. Staals1
1Wageningen University, Wageningen, The Netherlands 2University of Texas, Austin, United States 3Scope Biosciences, Wageningen, The Netherlands 4TNO, Zeist, The Netherlands 5University Medical Center, Utrecht, The Netherlands 6Delft University of Technology, Delft, The Netherlands 7RIKEN, Sayo, Japan 
0
5.20 pm (GMT+2)

Break


3
5.30 pm (GMT+2)

The arm race in Streptococcus thermophilus,
the ups and downs of CRISPR-Cas systems

Sylvain Moineau
Université Laval, Canada
4
6.00 pm (GMT+2)

Regulation of CRISPR-Cas9 systems by natural single-guide RNAs

R. Workman, J. Modell*
Molecular Biology & Genetics, Johns Hopkins School of Medicine, Baltimore, United States 
0
6.20 pm (GMT+2)

Break


5
6.30 pm (GMT+2)

Heritable Human Genome Editing: Science, Ethics and the Art of Policymaking

Francoise Baylis
Dalhousie University, Canada
0
7.00 pm (GMT+2)

Panel Discussion: Altering the Human genome

F. Baylis, K. Davies, W. Isaacson, C. Zimmer
Wednesday, June 2, 2021
Session 2
4.00 pm-8.00 pm (GMT+2)
Chair: White Malcolm, St Andrews University, United Kingdom
6
4.00 pm (GMT+2)

Structure and dynamics of CRISPR in natural populations

Rachel Whitaker
University of Illinois Urbana-Champaign, United States
7
4.30 pm (GMT+2)

Unique features of CRISPR spacer acquisition by the Type III-A system of Streptococcus thermophilus

S. Garrett*1, X. Zhang2, M. Terns2-3
1Genetics and Genome Sciences, University of Connecticut Health Center, Farmington, United States 2Department of Biochemistry and Molecular Biology, University of Georgia, Athens, United States 3Department of Microbiology, University of Georgia, Athens, United States 
0
4.50 pm (GMT+2)

Break


8
5.00 pm (GMT+2)

Vagaries of CRISPR evolution: crossing the boundary between classes and moonlighting as regulators

Eugene Koonin
National Center for Biotechnology Information, NIH, United States
9
5.30 pm (GMT+2)

Introducing Cas-beta: a new family of compact class 2 CRISPR associated nucleases

G. Gasiunas*1, T. Urbaitis1-2, JK. Young3, Z. Hou3, S. Paulraj3, E. Godlauskaite1, M. Grusyte1, E. Schildkraut4, C. Alarcon3, ND. Chilcoat3, BG. Robb4, V. Siksnys1-2
1CasZyme, Vilnius, Lithuania 2Institute of Biotechnology, Vilnius University, Vilnius, Lithuania 3Department of Molecular Engineering, Corteva Agriscience, Johnston, IA, United States 4New England Biolabs, Ipswich, MA, United States 
0
5.50 pm (GMT+2)

Break


10
6.00 pm (GMT+2)

Roseburia intestinalis resistance to phage predation by CRISPR spacer acquisition in the mouse gut

Marianne De-Paepe
INRA, France
11
6.30 pm (GMT+2)

Positioning type IV structures and functions within class 1 CRISPR Cas systems

R. Jackson
Jackson Lab, Utah State University, Logan, United States 
0
6.50 pm (GMT+2)

Break


0
7.00 pm (GMT+2)

Short-talks / Posters


Thursday, June 3, 2021
Session 3
10.00 am-2.00 pm (GMT+2)
Chair: Bernheim Aude, INSERM, France
12
10.00 am (GMT+2)

Characterization of natural and engineered Cas12 variants

John van der Oost
Wageningen University, The Netherlands
13
10.30 am (GMT+2)

A membrane-associated DHH-DHHA1 nuclease degrades type III CRISPR second messenger

R. Zhao, Y. Yang, W. Han*
Huazhong Agricultural University, Wuhan, China 
0
10.50 am (GMT+2)

Break


14
11.00 am (GMT+2)

Class 2 CRISPR-Cas nucleases: DNA cleavage in vitro and in cells

Virgis Siksnys
Vilnius University, Lithuania
15
11.30 am (GMT+2)

Mapping metabolite – transcription factor interactions at a genome-scale using CRISPRi knockdown library screenings

M. Kuntz*1-2, S. Donati3, V. Pahl1, T. Glatter2, JV. Gomes-Filho4, L. Randau4, H. Link1
1Bacterial Metabolomics, CMFI - IMIT University Tübingen, Tübingen, Germany 2Max Planck Institute for Terrestrial Microbiology, Marburg, Germany 3The Novo Nordisk Foundation Center for Biosustainability, Lyngby, Denmark 4University Marburg, Marburg, Germany 
0
11.50 am (GMT+2)

Break


16
12.00 pm (GMT+2)

Sequence-specific toxicity of dCas9 in bacteria

T. Grébert*, W. Rostain, P. Thiel-Pizzaro, G. Thinsele, L. Cui, D. Bikard
Synthetic Biology Group, Microbiology Department, Institut Pasteur, Paris, France 
17
12.20 pm (GMT+2)

A gate and clamp regulate sequential DNA strand cleavage by CRISPR-Cas12a

MM. Naqvi, L. Lee, OE. Torres Montaguth, M. Szczelkun*
Biochemistry, University of Bristol, Bristol, United Kingdom 
0
12.40 pm (GMT+2)

Break


0
12.50 pm (GMT+2)

Short-talks / Posters


Friday, June 4, 2021
Session 4
4.00 pm-8.00 pm (GMT+2)
Chair: Li Hong,
18
4.00 pm (GMT+2)

Exploration of Biological Diversity

Feng Zhang
Broad Institute of MIT and Harvard, Cambridge, United States
19
4.30 pm (GMT+2)

Development and Application of Minimal-PAM CRISPR-Cas Enzymes

K. Christie, R. Walton, M. Whittaker, B. Kleinstiver*
Mass General Hospital and Harvard Medical School, Boston, United States 
0
4.50 pm (GMT+2)

Break


20
5.00 pm (GMT+2)

Phase-dependent CRISPR evolution

Blake Wiedenheft
Montana State University, United States
21
5.30 pm (GMT+2)

The High-resolution Mechanism for Cas4-assisted PAM-selection and directional spacer acquisition in CRISPR-Cas

C. Hu1, C. Almendros2-3, K. Nam4, A. Costa2-3, J. Vink2-3, A. Haagsma2-3, S. Bagde1, S. Brouns2-3, A. Ke*1
1Department of Molecular Biology and Genetics, Cornell University, Ithaca, United States 2Department of Bionanoscience, Delft University of Technology, Delft, The Netherlands 3Kavli Institute of Nanoscience, Delft, The Netherlands 4Departmentof Life Science, Pohang University of Science and Technology, Pohang, South Korea 
0
5.50 pm (GMT+2)

Break


22
6.00 pm (GMT+2)

Determinants and consequences of CRISPR-Cas specificity in host-pathogen co-evolution

Dipali Sashital
Iowa State University, United States
23
6.30 pm (GMT+2)

Targeted DNA Editing Within Microbial Communities

B. Rubin*1, B. Cress1, S. Diamond1, A. Crits-Christoph1, H. Shivram1, C. He1, M. Zhou1, R. Sachdeva1, N. Krishnappa1, K. Tang1, D. Smock1, S. Smith1, M. Xu1, R. Barrangou2, A. Deutschbauer3, J. Banfield1, J. Doudna1
1Innovative Genomics Institute, UC Berkeley, Berkeley, United States 2Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, United States 3Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, United States 
0
6.50 pm (GMT+2)

Free slot / Spatial chat networking


Monday, June 7, 2021
Session 5
4.00 pm-8.00 pm (GMT+2)
Chair: Sontheimer Erik, UMass Medical School, United States
24
4.00 pm (GMT+2)

Functional Interplay Between CRISPR-Cas and Transposable Elements

Samuel Sternberg
Columbia University, United States
25
4.30 pm (GMT+2)

Harnessing diverse compact CRISPR-Cas3 for long-range genome engineering

R. Tan1, R. Krueger1, M. Gramelspacher1, X. Zhou1, Y. Liu1, A. Ke2, Z. Hou1, Y. Zhang*1
1Dept of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Mi, United States 2Dept. of Molecular Biology and Genetics, Cornell University, Ithaca, Ny, United States 
0
4.50 pm (GMT+2)

Break


26
5.00 pm (GMT+2)

Complex CRISPR-Cas and anti-CRISPR interactions in archaeon Sulfolobus

Xu Peng
University of Copenhagen, Denmark
27
5.30 pm (GMT+2)

Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells

P. Donohoue*1, M. Pacesa2, E. Lau1, B. Vidal1, A. Owen1, C. Fuller1, K. Baek1, E. Slorach1, B. Kohrs1, J. Gibson1, M. Toh1, M. Van Overbeek1, L. Banh1, T. Rotstein1, M. Irby1, B. Nyer1, A. May1, M. Jinek2, P. Cameron1
1Caribou Biosciences, Berkeley, United States 2Department of Biochemistry, University of Zurich, Zurich, Switzerland 
0
5.50 pm (GMT+2)

Break


28
6.00 pm (GMT+2)

Expanding the functional repertoire of CRISPR gene editing

David A. Scott
Arbor Biotechnologies, United States
29
6.30 pm (GMT+2)

Deciphering Off-Target Effects in CRISPR-Cas9 through Accelerated Molecular Dynamics

P. Arantes*1, C.G. Ricci2, J.S. Chen3, Y. Miao4, M. Jinek5, J.D. Doudna6, J.A. Mccammon2, G. Palermo1
1Bioengineering, University California, Riverside, Riverside, United States 2Chemistry and Biochemistry Department, University California, San Diego, San Diego, United States 3Mammoth Biosciences: South San Francisco, San Francisco, United States 4Department of Molecular Biosciences, The University of Kansas, Lawrence, United States 5Department of Biochemistry, University of Zurich, Zurich, Switzerland 6Department of Chemistry, University California, Berkeley, Berkeley, United States 
0
6.50 pm (GMT+2)

Break


0
7.00 pm (GMT+2)

Panel: Career Paths in CRISPR

April Pawluk
Harvard Chan Microbiome in Public Health Center, Boston, United States
Tuesday, June 8, 2021
Session 6
4.00 pm-8.00 pm (GMT+2)
Chair: Jinek Martin, University of Zurich, Switzerland
30
4.00 pm (GMT+2)

An anti-CRISPR that Rips Apart the Type I-F CRISPR-Cas System

Alan Davidson
University of Toronto, Toronto, Canada
31
4.30 pm (GMT+2)

Anti-CRISPR Proteins are Associated with Ribbon-Helix-Helix DNA-binding Domains

C. Trost*1, R. Wilson2, J. Wang3-4, B. Garcia1, Y. Hidalgo-Reyes1, W.T. Lu2, M. Mejdani5, Y. Wang3-4, A. Davidson1-2
1Department of Molecular Genetics, University of Toronto, Toronto, Canada 2Department of Biochemistry, University of Toronto, Toronto, Canada 3National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China 4Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China 5Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada 
0
4.50 pm (GMT+2)

Break


32
5.00 pm (GMT+2)

Impact of anti-CRISPR on the ecology and evolution of phages and bacteria

Anne Chevallereau
CNRS, France
33
5.30 pm (GMT+2)

Single Cell Variability of CRISPR-Cas Interference and Adaptation

R. Mckenzie*1-2, E. Keizer3, J. Vink1, C. Fleck4, S. Tans2-1, S. Brouns1
1Department of Bionanoscience, Delft University of Technology, Delft, The Netherlands 2AMOLF, Amsterdam, The Netherlands 3Biometris, Department of Mathematical and Statistical Methods, Wageningen University, Wageningen, The Netherlands 4Freiburg Center for Data Analysis and Modeling, University of Freiburg, Freiburg, Germany 
0
5.50 pm (GMT+2)

Break


34
6.00 pm (GMT+2)

tRNA cleavage by Cas13a protein from Leptotrichia shahii

E. Semenova*1, I. Jain1-2, M. Kolesnik2, K. Severinov1-2
1Waksman Institute, Rutgers University, Piscataway, United States 2Skolkovo Institute of Science and Technology, Skolkovo, Russia 
35
6.20 pm (GMT+2)

AcrVA2 inhibits Cas12a biogenesis via translation-dependent mRNA degradation

N. Marino*, J. Bondy-Denomy
Microbiology and Immunology, Bondy-Denomy Lab, University of California San Francisco, San Francisco, United States 
0
6.40 pm (GMT+2)

Break


0
6.50 pm (GMT+2)

Short-talks / Posters


Wednesday, June 9, 2021
Session 7
10.00 am-2.00 pm (GMT+2)
Chair: Fineran Peter, University of Otago, New Zealand
36
10.00 am (GMT+2)

Non-canonical crRNAs derived from host transcripts enable multiplexable RNA detection

Cynthia Sharma
Wuerzburg University, IMIB, Germany
37
10.30 am (GMT+2)

Bacteriostatic antibiotics promote the evolution of CRISPR-Cas immunity against phage in a human pathogen

T. Dimitriu*1, E. Kurilovich2, U. Lapinska1, S. Pagliara1, M. Szczelkun3, E. Westra1
1University of Exeter, Penryn, United Kingdom 2Skolkovo Institute of Science and Technology, Moscow, Russia 3University of Bristol, Bristol, United Kingdom 
0
10.50 am (GMT+2)

Break


38
11.00 am (GMT+2)

So, who's defending whom from what?

Eduardo Rocha
Institut Pasteur, France
39
11.30 am (GMT+2)

CRISPR-Cas is associated with fewer AMR genes in bacterial human pathogens

E. Pursey*1, T. Dimitriu1, FL. Paganelli2, ER. Westra1, S. Van Houte1
1University of Exeter, Penryn, United Kingdom 2UMC Utrecht, Utrecht, The Netherlands 
0
11.50 am (GMT+2)

Break


40
12.00 pm (GMT+2)

New insights from CRISPR spacer mapping

Stan Brouns
TU Delft, The Netherlands
41
12.30 pm (GMT+2)

Cas13 immune defense and autoimmunity is dictated by a threshold for RNA target levels

E. Vialetto*1, Y. Yu1, S. P. Collins2, L. Barquist1-3, C. L. Beisel1-3
1Helmholtz Institut for RNA-based infection research (HIRI), Würzburg, Germany 2Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, United States 3Medical Faculty, University of Würzburg, Würzburg, Germany 
0
12.50 pm (GMT+2)

Break


0
1.00 pm (GMT+2)

Short-talks / Posters


Thursday, June 10, 2021
Session 8
4.00 pm-7.00 pm (GMT+2)
Chair: Pawluk April, Harvard Chan Microbiome in Public Health Center, United States
42
4.00 pm (GMT+2)

New genetic systems that protect bacteria from phage

Rotem Sorek
Weizmann Institute of Science, Israel
43
4.30 pm (GMT+2)

Antiviral activity of bacterial TIR domains via signaling molecules that trigger cell death

G. Ofir*1, E. Herbst1, M. Baroz1, D. Cohen1, A. Millman1, N. Tal1, S. Doron1, DBA. Malheiro2, S. Malitsky3, G. Amitai1, R. Sorek1
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel 2MS-Omics, Vedbaek, Denmark 3Life Science Core Facilities, Weizmann Institute of Science, Rehovot, Israel 
0
4.50 pm (GMT+2)

Break


44
5.00 pm (GMT+2)

Quorum sensing anti-activation in the phage-host evolutionary arms race

Karen Maxwell
University of Toronto, Canada
45
5.30 pm (GMT+2)

Characterizing a novel anti-phage immune system in Staphylococcus epidermidis

N. Bari*1, L. Chou-Zheng1, K. Cater2, V. Dandu2, A. Thomas2, A. Hatoum-Aslan1
1Microbiology, University of Illinois at Urbana-Champaign, Urbana, United States 2Biological Science, University of Alabama, Tuscaloosa, United States 
0
5.50 pm (GMT+2)

Break


46
6.00 pm (GMT+2)

Multiplexed genome editing of poplar for improved fiber processing and sustainable forestry

J. Wang*1-2-3, D. Sulis2, M. Matthews2, C. Williams2, R. Shi2, C. Yang2, B. Marques2, S. Tunlaya-Anukit2, H.C. Chen2, Q. Li4, J. Liu2, C.Y. Lin2-5, P. Naik2, Y.H. Sun6, P. Loziuk2, T.F. Yeh7, H. Kim8, E. Gjersing5, T. Shollenberger5, R. Bing2, C. Straub2, P.A. Khatibi2, C. Shuford2, J. Song2, Z. Miller2, Y.Y. Huang2, C.W. Edmunds2, B.G. Liu3, Y. Sun3, Y.C.J. Lin2-7, W. Li2-3, H. Chen2, I. Peszlen2, J.J. Ducoste2, J. Ralph8, R.M. Kelly2, H.M. Chang2, H. Jameel2, D.C. Muddiman2, M.F. Davis5, C. Smith2, F. Isik3, R.R. Sederoff1-2-3, V.L. Chiang1-2-3, R. Barrangou1-2
1TreeCo, NC State University, Raleigh, United States 2North Carolina State University, Raleigh, United States 3Northeast Forestry University, Harbin, China 4Chinese Academy of Forestry, Beijing, China 5National Renewable Energy Laboratory, Golden, United States 6National Chung-Hsing University, Taichung, Taiwan 7National Taiwan University, Taipei, Taiwan 8University of Wisconsin, Madison, United States 
47
6.20 pm (GMT+2)

New mechanisms and applications of CRISPR-Cas systems

Jennifer Doudna
UC Berkeley, United States
0
6.50 pm (GMT+2)

Poster winner announcement & closing remarks


 

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